Abstract
Objective To explore the microRNA differential expression profile between Ⅰ-Ⅲ papillary thyroid carcinoma (PTC) and peritumoral tissues so as to provide rationals for the role of microRNA in the pathogenesis of PTC.Methods Samples of stage Ⅰ,Ⅱ and Ⅲ PTC and peritumoral tissues (n =1 for each) were collected.The expression levels of microRNAs were examined by using microRNA microarray chip.MicroRNAs that were sequentially upregulated or downregulated with clinical staging were further analyzed.Some of miRNAs microarray results were verified by real-time quantitative polymerase chain reaction (real-time PCR).Some potential miRNAs regulating target genes were forecasted by using function prediction software,including their preliminary biological functions.Results Compared with peritumoral tissues,20 microRNAs exhibited significant differential expression in PTC.In addition,8 microRNAs were up-regulated sequentially in stage Ⅰ-Ⅲ PTC tissues,and 5 microRNAs were down-regulated sequentially.Conclusion There is a differential expression of microRNA between stage Ⅰ-Ⅲ PTC and peritumoral tissues,which may be related with the occurrence and metastasis of PTC. Key words: Papillary thyroid carainoma; MicroRNAs; Chip technology
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