Differential expression profile of long non-coding RNAs from Gr-1+ CD11b+ myeloid cells in response to sepsis in mice (INM2P.424)

Abstract

Abstract Background: Emerging evidence shows that small noncoding RNAs are involved in the immune response to sepsis. While, whether long noncoding RNAs (lncRNAs) are associated with the immune response to sepsis is currently unexplored. So we performed a global expression profile of lncRNAs from myeloid-derived suppressor cells (MDSCs), which are an important population that mediate immune suppression in response to sepsis, defined by the cell-surface phenotype Gr-1+ and CD11b+. Methods: Sepsis was induced by cecal ligation puncture (CLP) in mice. MDSCs were sorted from spleens by magnetic selection and FACS 72 h after CLP or sham surgery. The Arraystar lncRNA microarray was used to profile the genome-wide lncRNA expression. The expression pattern and classification of lncRNAs were evaluated by bioinformatics. Results: The microarray provided 16,551 lncRNAs in the septic group and 17,381 in the sham group. After sepsis, among the lncRNAs expressed in both groups, 139 were upregulated and 268 downregulated by ≥ 1.5-fold compared with sham. Nearly half of the differentially-expressed lncRNAs were intergenic RNAs. qRT -PCR confirmed the lncRNA expression pattern of microarray hybridization. Conclusions: The present study provided the first global profile of lncRNA expression in response to sepsis. The expression pattern of lncRNAs in MDSCs was affected by sepsis. Future researches are needed to determine candidate lncRNAs as biomarkers or therapeutic targets for sepsis.