Abstract
An oxygen sensitive mutant of Azorhizobium caulinodans strain IRBG 46 in which N2 fixation ability was affected, was previously isolated by NTG mutagenesis. Now, the mutation has been shown to affect H2- uptake hydrogenase (Hup) activity under symbiotic conditions. However, free living Hup activity remained unaffected. Thus the mutant is Hup- under symbiotic conditions and Hup+ under free living condtions. A possible regulatory link between N2 fixation and H2 uptake system has been discussed.
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