Differential Expression of Two Prolactin and Growth Hormone Genes during Early Development of Tilapia ( Oreochromis mossambicus) in Fresh Water and Seawater: Implications for Possible Involvement in Osmoregulation during Early Life Stages

Abstract

The possible involvement of prolactin (PRL) and growth hormone (GH) in osmoregulation during early life stages of the tilapia ( Oreochromis mossambicus) was examined by in situ hybridization and immunocytochemistry using synthetic oligonucleotide probes and homologous antisera to two tilapia PRLs (PRL 188 and PRL 177) and GH. Hybridization signals for PRL 188 mRNA were detected for the first time in newly hatched larvae (5 days after fertilization), and were significantly greater in larvae in fresh water (FW) than those in seawater (SW) until 10 days after hatching. PRL 177 mRNA was detected in the pituitary of embryos 1 day before hatching. Although PRL 177 gene expression in the embryo and newly hatched larvae in FW was not significantly different from those in SW, the expression was significantly greater in FW than in SW from Day 2 until Day 10. Hybridization signals for GH mRNA were first detected in newly hatched larvae. No significant differences in GH mRNA expression were observed between larvae in FW and those in SW. A stronger immunoreaction, a significantly larger PRL cell size, and a pituitary area containing PRL cells were observed in larvae hatched and maintained in FW, in those transferred from SW to FW, compared to larvae hatched and maintained in SW, and in those transferred from FW to SW. No significant difference was observed in the activity of GH cells between larvae in FW and those in SW. These results suggest that both PRLs are involved importantly in FW adaptation, whereas GH does not seem to play a critical role in osmoregulation during early stages of tilapia.