Abstract
BackgroundThe predominant genotype of Toxoplasma in China is the Chinese 1 (ToxoDB#9) lineage. TgCtwh3 and TgCtwh6 are two representative strains of Chinese 1, exhibiting high and low virulence to mice, respectively. Little is known regarding the virulence mechanism of this non-classical genotype. Our previous RNA sequencing data revealed differential mRNA levels of TgMIC1 in TgCtwh3 and TgCtwh6. We aim to further confirm the differential expression of TgMIC1 and its significance in this atypical genotype.MethodsQuantitative real-time PCR was used to verify the RNA sequencing data; then, polyclonal antibodies against TgMIC1 were prepared and identified. Moreover, the invasion and proliferation of the parasite in HFF cells were observed after treatment with TgMIC1 polyclonal antibody or not.ResultsThe data showed that the protein level of TgMIC1 was significantly higher in high-virulence strain TgCtwh3 than in low-virulence strain TgCtwh6 and that the invasion and proliferation of TgCtwh3 were inhibited by TgMIC1 polyclonal antibody.ConclusionDifferential expression of TgMIC1 in TgCtwh3 and TgCtwh6 may explain, at least partly, the virulence mechanism of this atypical genotype.Graphical
Highlights
The predominant genotype of Toxoplasma in China is the Chinese 1 (ToxoDB#9) lineage
The predominant genotype of T. gondii in China is Chinese I (ToxoDB#9) lineage (> 79% based on 60 isolates) [6]
Quantitative real-time PCR was used to identify the mRNA expression of TgMIC1, TgMIC4 and TgMIC6 in TgCtwh3 and TgCtwh6 strains
Summary
The predominant genotype of Toxoplasma in China is the Chinese 1 (ToxoDB#9) lineage. TgCtwh and TgCtwh are two representative strains of Chinese 1, exhibiting high and low virulence to mice, respectively. Toxoplasma gondii is an intracellular parasite belonging to the phylum Apicomplexa that infects a wide range of warm-blooded vertebrates, including approximately one-third of humans [1]. In most hosts, this intracellular protozoan parasite escapes immunological surveillance and can cross the blood-brain and blood-retina barrier reaching alleged immune privileged regions to establish a. The predominant genotype of T. gondii in China is Chinese I (ToxoDB#9) lineage (> 79% based on 60 isolates) [6]. Our previous studies have demonstrated that the isolates of Chinese I vary in their virulence in mice; among these, TgCtwh exhibits high virulence, whereas TgCtwh exhibits low virulence [6,7,8]
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