Abstract
BackgroundRNA binding motif 5 (RBM5) is a tumor suppressor gene that modulates apoptosis through the regulation of alternative splicing of apoptosis-related genes. This study aimed to detect RBM5 expression in non-small cell lung cancer (NSCLC) and to associate RBM5 expression with clinicopathological data from NSCLC patients and EGFR and KRAS expression to better understand the potential role of RBM5 in NSCLC.MethodSemi-quantitative reverse transcription-polymerase chain reaction (RT-PCR) and Western blotting were performed to detect expression of mRNA and protein, respectively, of RBM5, EGFR and KRAS in 120 paired non-tumor and tumor samples of NSCLC.ResultsThe data showed that expression of RBM5 mRNA and protein was significantly reduced in NSCLC compared to normal tissues, whereas expression of both EGFR and KRAS genes was increased in NSCLC compared to normal tissues. Furthermore, the reduced RBM5 protein expression correlated with smoking status, tumor stage and lymph node metastasis of NSCLC, while overexpression of EGFR and KRAS proteins correlated with tumor stage and lymph node metastasis of NSCLC. Overexpression of KRAS protein was more frequent in smokers with NSCLC. In addition, expression of RBM5 mRNA and protein was negatively correlated with expression of EGFR and KRAS mRNA and protein in NSCLC tissues.ConclusionThis study suggests further evaluation of RBM5 expression is warranted for use of RBM5 as a biomarker for NSCLC patients.
Highlights
Lung cancer is a significant worldwide health problem, accounting for more than 1.5 million new cases and 1.3 million cancer-related deaths annually [1,2]
Differential expression of RNA binding motif 5 (RBM5) mRNA and protein in nonsmall cell lung cancer (NSCLC) In this study, we first detected the expression of RBM5 mRNA and protein in 120 paired NSCLC and adjacent normal tissue specimens
By comparison of normal and tumor expression of RBM5 mRNA and protein at a ratio of 2.0 as a cutoff point we found that expression of RBM5 mRNA and protein was significantly reduced in NSCLC vs. the non-tumor tissues (P = 0.037 and P = 0.03, respectively)
Summary
Lung cancer is a significant worldwide health problem, accounting for more than 1.5 million new cases and 1.3 million cancer-related deaths annually [1,2]. It remains to be defined how tobacco smoke and other risk factors cause the development of NSCLC, further study of the underlying mechanisms responsible for NSCLC development and progression is truly needed to provide novel strategies in early detection and effective control of this deadly disease. Upon binding of specific ligands, such as epidermal growth factor and transforming growth factor-α, the receptor forms homodimers, leading to receptor autophosphorylation and activation of the signal cascade. This results in changes in expression of different genes that are crucial to tumor progression, including tumor growth, resistance to apoptosis, invasion, and angiogenesis [8]. This study aimed to detect RBM5 expression in nonsmall cell lung cancer (NSCLC) and to associate RBM5 expression with clinicopathological data from NSCLC patients and EGFR and KRAS expression to better understand the potential role of RBM5 in NSCLC
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More From: Journal of Experimental & Clinical Cancer Research
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