Differential expression of P63, P27, P57, Ki-67, and CD146 in hydropic and molar pregnancies.

Abstract

To investigate the expressions of immunohistochemical markers P63, P27, P57, Ki-67 and CD146 in hydropic and molar specimens in order to explore their role in the pathogenesis of molar gestations. The retrospective study was conducted at the Kırıkkale Medical University, Turkey, and comprised data from 2011 to 2018 related to patients with a definitive pathological diagnosis of hydropic abortus, complete hydatidiform moles and partial hydatidiform moles. Immunoreactivity using antibodies against P63, P27, P57, Ki-67 and CD146 was scored by evaluating the percentage of distinctly stained cells. Data was analysed using SPSS 15. Of the 37 specimens, 10(27%) were hydropic abortus, 17(46%) partial hydatidiform moles and 10(27%) complete hydatidiform moles. Patients with complete hydatidiform moles severe cytologic atypia in CD146-positive extravillous trophoblastic column and florid syncytiotrophoblast proliferation. P57 immunostaining was negative in 9(90%) patients with complete hydatidiform moles, whereas all patients in the two other groups showed positive immunostaining, and they also showed P63 and Ki-67 overexpression in cytotrophoblasts. P27 was expressed in differentiated, non-dividing syncytiotrophoblasts but did not yield any diagnostic aid. The proliferative activity location varied between molar and nonmolar pregnancies.