Abstract
BackgroundPorcine parvovirus (PPV), a member of the Parvoviridae family, causes great economic loss in the swine industry worldwide. MicroRNAs (miRNAs) are a class of non-protein–coding genes that play many diverse and complex roles in viral infections.FindingAiming to determine the impact of PPV infections on the cellular miRNAome, we used high-throughput sequencing to sequence two miRNA libraries prepared from porcine kidney 15 (PK-15) cells under normal conditions and during PPV infection. There was differential miRNA expression between the uninfected and infected cells: 65 miRNAs were upregulated and 128 miRNAs were downregulated. We detected the expression of miR-10b, miR-20a, miR-19b, miR-181a, miR-146b, miR-18a, and other previously identified immune-related miRNAs. Gene Ontology analysis and KEGG function annotations of the host target genes suggested that the miRNAs are involved in complex cellular pathways, including cellular metabolic processes, immune system processes, and gene expression.ConclusionsThese data suggest that a large group of miRNAs is expressed in PK-15 cells and that some miRNAs were altered in PPV-infected PK-15 cells. A number of microRNAs play an important role in regulating immune-related gene expression. Our findings should help with the development of new control strategies to prevent or treat PPV infections in swine.
Highlights
Porcine parvovirus (PPV), a member of the Parvoviridae family, causes great economic loss in the swine industry worldwide
These data suggest that a large group of miRNAs is expressed in porcine kidney 15 (PK-15) cells and that some miRNAs were altered in PPV-infected PK-15 cells
A number of microRNAs play an important role in regulating immune-related gene expression
Summary
Porcine parvovirus (PPV), a member of the Parvoviridae family, causes great economic loss in the swine industry worldwide. MicroRNAs (miRNAs) are a class of non-protein–coding genes that play many diverse and complex roles in viral infections. Porcine parvovirus (PPV) is a major cause of reproductive failure in swine (Sus scrofa, ssc), where infection is characterized by early embryonic death, stillbirths, fetal death, and delayed return to estrus [1]. Even though inactivated and attenuated vaccines are widely used, the PPV-associated diseases cause serious economic losses to the swine industry worldwide [3]. Determining the consequences of PPV infections on cellular gene regulatory networks is urgent. Sichuan Agricultural University, Wenjiang, Chengdu, China miRNAs are involved in post-transcriptional regulation of gene expression in animals, plants, and some DNA viruses. An increasing number of researchers have focused on the relationship between viruses and miRNAs
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
