Differential expression of Fos protein in the rat brain induced by performance of avoidance or escape in the elevated T-maze

Abstract

Analysis of Fos protein expression was used to map brain areas activated by exposure of male Wistar rats to the elevated T-maze, an animal model of anxiety where tasks of inhibitory avoidance or one-way escape can be separately performed. The apparatus consists of three elevated arms — one enclosed and two open. In the inhibitory avoidance task — considered to represent learned fear — the time taken by rats to leave from the enclosed arm in three consecutive trials is measured. One-way escape task is measured by recording the time taken by animals to withdraw from the open arm and is thought to reflect innate fear. Control animals were placed three times at the end of the transversal arm of a T-maze composed of three enclosed arms and withdrawal latencies from this arm was similarly measured. Performance of avoidance task increased Fos-like immunoreactivity in the medial amygdaloid nucleus, in the anterior hypothalamic nucleus and in the median raphe nucleus. In contrast, performance of escape task enhanced Fos-like immunoreactivity in the basolateral amygdaloid nucleus and in the dorsal periaqueductal gray matter of the mesencephalon. Both behavioural tasks promoted an increase in Fos-like immunoreactivity in the paraventricular nucleus of the thalamus and in the dorsomedial hypothalamic nucleus. Therefore, the obtained results indicate that different sets of brain structures were, respectively, activated by inhibitory avoidance and one-way escape. This evidence supports the original hypothesis that two types of fear/anxiety are generated in the elevated T-maze.