Differential expression of exosomal miRNAs between androgen-dependent prostate cancer cell and androgen-independent prostate cancer cell

Abstract

Objective Studying the differential expression of exosomal miRNAs between androgen-dependent prostate cancer cell and androgen-independent prostate cancer cell, in order to further elucidate the mechanism of androgen-independent prostate cancer and find new targets for its treatment. Methods Prostate cancer androgen-dependent LNCaP cell and prostate cancer androgen-independent LNCaP-AI+F cell (induced by androgen and flutamide) were selected as the study subjects. Illumina HiSeqTM 2500 was used to perform high-throughput sequencing between the two groups. The differentially expressed exosomal miRNAs was verified by quantitative real-time PCR(qRT-PCR). The difference was statistically significant by t-test. Results Through the analysis of high-throughput sequencing results, thirteen molecules were screened increased in extracellular exosomes of the androgen-independent cell, including miR-7-5p, let-7a-5p, miR-375, miR-423-3p, miR-378a-3p, and miR-92a-3p, etc. Among them, miR-7-5p was verified by quantitative real-time PCR to be up-regulated by 19.52-fold (t=9.857, P=0.001). Conclusion Differentially expressed exosomal miRNAs may predict the development of androgen-independent prostate cancer and may further regulate the development of androgen-independent prostate cancer. Key words: Prostatic neoplasms; Exosomes; microRNAs; High-throughput nucleotide sequencing