Abstract
This study provides the first empirical evidence that Lysinibacillus sphaericus C3-41 has chromosomal- and plasmid-borne bin operon and both are necessary for full toxicity against mosquito larvae, of which the plasmid-borne one contributes more to the production and activity of the binary toxin than the chromosomal-born one. The study provides an important clue for the evolution and application of mosquitocidal L. sphaericus.
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