Differential expression of corticotropin releasing factor and urocortins in the Schistosoma mansoni‐infected mouse ileum (214.2)

Abstract

Corticotropin releasing factor (CRF) and urocortins (UCNs) are important ligands in the CRF signaling pathways, most well‐known from the hypothalamic‐pituitary‐adrenal stress axis. However, peripheral CRF signaling has profound effects on gastrointestinal functions. Despite the potential of the murine animal model in elucidating this complexly balanced pathway through genetic manipulation, little is known regarding the expression of CRF and UCNs in the intestinal wall of the mouse. This study therefore investigates the cellular localization of CRF and UCNs in the ileum and examines how this cellular expression is altered in intestinal schistosomiasis. The results show a diverse expression of CRF and UCNs in the enteric nervous system, granulocytes and the ileal musculature. Of special interest was the de novo expression of UCN3 in a majority of submucous vasodilator/secretomotor neurons, possibly strengthening CRF2‐mediated effects during inflammation. In conclusion, we outline for the first time the ileal expression of CRF signaling ligands in the mouse, and point to important changes of this signaling system in intestinal inflammation.Grant Funding Source: Supported by a grant of the Flemish Organization for Scientific Research (FWO G.0179.08)