Differential Expression of ACC Synthase Genes in Response to Stress

Abstract

Ethylene production is involved in many plant physiological processes including stress responses and is frequently associated with foliar senescence. Ethylene emission is a common plant response to many biotic and abiotic stresses. We have cloned two ACC synthase cDNAs (OIP-1, PAC-1) from the leaves of ozone treated Solanum tuberosum L. plants. Plants treated with ozone produced ethylene within 1 hour following treatment initiation. Levels continued to increase reaching a peak after 2 h. PAC-1 was expressed after 1 hour reaching a maximum by 2 hours and showed a marked decline after 4 h. OIP-1 was first expressed after 2 hours and high levels of expression continued up to 4 hours following treatment initiation. Leaves treated with CuCl2 produced high levels of ethylene within 0.5 hour after treatment initiation. Ethylene levels continued to increase reaching a peak after 2 hours with no change after 4 h. PAC-1 was expressed after 0.5 hour reaching a peak at 1 hour and showed a progressive decline from 2 to 4 h. However, OIP-1 expression was first detected 2 hours following treatment initiation and high levels of expression continued up 4 h. Leaves exposed to Alternaria solani produced increased levels of ethylene 1 day following inoculation reaching a peak after 3 days. PAC-1 was expressed at a low level 1 day after inoculation and expression remained constant for the duration of the experiment, whereas, OIP-1 was not expressed until day 4.