Abstract
Pitaya is a burgeoningly tropical fruit, there are two common varieties, red peel with red pulp and red peel with white pulp. To investigate the reason of different colors in two kinds of pitaya, explore differencially expressed genes (DEGs), in this study, we profiled transcriptome in flesh of two varieties (Hylocereu polyrhizus and Hylocereu undatus) in green stage and mature stage respectively. 53 240 reads with high quality were obtained. Analysing the DEGs, we found there were bigger differences in different stage rather than different variety. Gene ontology (GO) functional analysis and KEGG pathway analysis were adopted in R1 VS R3 and W3 VS W3. Through GO analysis, we found many genes enriched in protein binding transcription factor activity in molecule founction. Through KEGG pathway analysis, we found most genes were enriched in biosynthesis of secondary metabolism, amino acid and nucleic acid metabolism, and tyrosine metabolism. In addintion, tyrosine was the precursor of betalaines. In conclusion, the development of Hylocereu polyrhizus accompanying by the synthesis of a large amount of betalaines, tyrosine played a key role as a precursor, and the synthesis of this pigment required a mass of amino acids, enzymes, and transcription factors. Betalaines synthesis pathway has not cleared yet, this study explored key genes related to betalaines synthesis and provided useful information for optimizing the betalaines synthesis pathway, which was the basis of later experiments.
Highlights
Pitaya is a plant belonging to Cactaceae Hylocereus
Through KEGG pathway analysis, we found most genes were enriched in biosynthesis of secondary metabolism, amino acid and nucleic acid metabolism, and tyrosine metabolism
The development of Hylocereu polyrhizus accompanying by the synthesis of a large amount of betalaines, tyrosine played a key role as a precursor, and the synthesis of this pigment required a mass of amino acids, enzymes, and transcription factors
Summary
1.1 Results of assembly of transcriptome data The transcriptome sequencing data of Hylocereu polyrhizus (R) and Hylocereu undatus (W) in green stage and mature stage were processed respectively. The final high-quality sequences assembled in the four samples were 5 914 708, 13 946 140, 14 364 654 and 5 296 884, respectively, and the assembly ratios of each sample were 91.13%, 91.52%, 91.81% and 93.42%, the final high-quality sequences were 5 914 708, 13 946 140, 14 364 654 and 5 296 884, respectively. The transcriptome data were spliced by Trinity software (Table 1). Through the statistical analysis of the length of the assembled sequence (Figure 1), excluding the head and tail, the length distribution of the sequence is uniform, which shows that the assembly result is reliable
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
