Abstract
A method is described for the determination of the individual levels of adrenaline, noradrenaline, dopamine, metanephrine and normetanephrine in urine. The amines present in the urine are adsorbed on a weak cation exchange resin (Amberlite IRC-50) and the catecholamines then eluted by a boric acid solution followed by metanephrine and normetanephrine with dilute sulfuric acid. The amines in each fraction are oxidised by iodine to the corresponding aminochromes which are then converted to their sodium bisulfite addition compounds. The levels of each of the five amines are calculated from measurements of the absorbances, at several wave-lengths, of the solutions of the bisulfite addition compounds. The average excretion rates of the free amines in urine, based on 41 determinations using this method are (in μg/h): A∗∗∗, 0.04; N.2.36; D.8.22; M, 1.25; NM, 8.55. The corresponding excretion rates (in μg/h) for the total amines ( i.e. free plus conjugated) are: A, 0.06; N, 4.69; D, 27.20; M, 3.65; N.M. 17.48.
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