Abstract
The effects of progesterone and 17β-estradiol on Ca 2+ signaling in in situ endothelial cells were investigated using front-surface fluorometry of fura-2-loaded strips of porcine aortic valve. Progesterone inhibited the thapsigargin-induced sustained [Ca 2+] i elevation (IC 50=33.9 μM, n=4), while 17β-estradiol added a transient [Ca 2+] i elevation. Progesterone and 17β-estradiol had no significant effect on the thapsigargin-induced [Ca 2+] i elevations in the absence of extracellular Ca 2+. A Mn 2+-induced decline of fluorescent intensity at 360 nm excitation was accelerated by thapsigargin. This acceleration was completely reversed by progesterone, but not by 17β-estradiol. Progesterone inhibited, and 17β-estradiol enhanced the endothelin-1 (ET-1)-induced [Ca 2+] i elevation, while both had no effect on the ET-1-induced Ca 2+ release observed in the absence of extracellular Ca 2+ or in the pertussis toxin-treated strips. Progesterone and 17β-estradiol thus had different effects on Ca 2+ signaling, especially on Ca 2+ influx, in endothelial cells.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callMore From: Biochimica et Biophysica Acta (BBA) - Molecular Cell Research
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
