Abstract
Abstract The effects of polylysine on the electron transfer from NADH to cytochrome c and ferricyanide in corn root plasma membrane vesicles were investigated. The presence of polylysine decreased the reduction rate of cytochrome e but slightly stimulated the rate of ferricyanide reduction in a pH 7.5 medium of low ionic strength. An increase in ionic strength eliminated the effects of polylysine on ferricyanide reduction but not the inhibition of cytochrome c reduction. Kinetic analyses indicated that in high ionic strength medium, polylysine inhibition appeared to assume a competitive model suggesting its binding to cytochrome c reduction site. In low ionic strength medium, a complex inhibition was observed. A study on the inhibitory efficiency of different size polylysines suggested that the reduction site of cytochrome c may best fit a polylysine with molecular weight close to 25,000. The results support the notion that the electron transfer from NADH to these two acceptors may involve two separated e...
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