Abstract

SummaryThe purpose of this study was to gain insight into the role of sulfhydryl (SH) groups in arterial receptors for vasoactive simulants. We studied the effects of p-chloromercuriphenyl sulfonate (PCMBS, 10-6 to 10-4 M), a potent and specific SH binding agent, on contractile responses produced with 10-5M epinephrine and 10-5M serotonin in helical strips of arterial smooth muscle prepared from tail arteries of the rat. Responses to epinephrine were rapidly, markedly, and reversibly inhibited with low concentrations of PCMBS. In contrast, responses to serotonin were slowly inhibited when high concentrations of PCMBS were tested. The inhibitory effect of PCMBS (10-5M) on epinephrine-induced contractions was prevented by 10-3 M cysteine. Alpha-adrenergic blockade with phentol-amine (10-4 or 5 × 10-6 M) virtually abolished responses to epinephrine and significantly decreased responses to serotonin. These findings are consistent with the view that arterial receptors for contractile responses to epinephrine a...

Talk to us

Join us for a 30 min session where you can share your feedback and ask us any queries you have

Schedule a call

Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.