Abstract
Macrophages (MΦ) and dendritic cells (DCs) are both pivotal antigen presenting cells capable of inducing specific cellular responses to inhaled mycobacteria, and thus, they may be important in the initiation of early immune responses to mycobacterial infection. In this study, we evaluated and compared the roles of murine splenic DCs and MΦs in immunity against Mycobacterium bovis Bacillus Calmette-Guérin (M.bovis BCG). The number of internalized rBCG-GFP observed was obviously greater in murine splenic MΦs compared with DCs, and the intracellular reactive oxygen species (ROS), inducible nitric oxide synthase (iNOS) and nitric oxide (NO) levels in MΦs were all higher than in DCs. DCs have a stronger capacity for presenting Ag85A peptide to specific T hybridoma and when the murine splenic MΦs were infected with BCG and rBCG::Ag85A, low level of antigen presenting activity was detected. These data suggest that murine splenic MΦs participate in mycobacteria uptake, killing and inducing inflammatory response, whereas the murine splenic DCs are primarily involved in specific antigen presentation and T cell activation.
Highlights
One of the major causes of death by infectious disease worldwide is Mycobacterium tuberculosis (M.tb) infection, which is an extraordinarily successful human pathogen [1]
A significant difference was observed in the number of ingested rBCG-GFP, which was higher in murine splenic MΦs compared with dendritic cells (DCs), and the mature
antigen presenting cells (APCs) in murine spleen may decrease in uptake of M.bovis BCG compared to monocyte derived MΦs and DCs
Summary
One of the major causes of death by infectious disease worldwide is Mycobacterium tuberculosis (M.tb) infection, which is an extraordinarily successful human pathogen [1]. A serial of microbicidal mechanisms will be activated after the MΦs were infected by M.tb, including phagolysosome fusion, respiratory burst, and the secretion of multiple proinflammatory cytokines, which control the growth of inhaled mycobacteria and activate additional recruited immune cells [5,6]. Both MΦs and DCs can process and present Ag to T cells, DCs are unique because of their role in priming the T cell response against many pathogens [7,8].
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
