Abstract
The sodium ionophore monensin was used as an inhibitor of Golgi function to study the secretory event in suspension-cultured sycamore cells. The morphological changes induced by monensin were recorded. Concurrent studies of the disturbance caused by the drug were carried out by biochemical, cytochemical and immunocytochemical techniques. Monensin induced accumulation of smooth and coated vesicles in the vicinity of the Golgi apparatus, swelling of Golgi cisternae and also provoked the formation of extracytoplasmic pockets between the plasma membrane and the cell wall. Cytochemical tests for polysaccharides and immunolocalization of secreted pectins, recognized by the monoclonal antibody JIM 7, were performed, and the results indicated that the biosynthesis and/or processing machinery of polysaccharides was affected by the drug
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