Abstract
This study evaluates the effect of Mg 2+ on the extramitochondrial hydrolysis of ATP and ADP by human term placental mitochondria (HPM) and submitochondrial particle (SMP). Extramitochondrial ATPase and ADPase activities were evaluated in the presence or absence of K +, and different oxidizable substrates. Mg 2+ increased both ATP and ADP hydrolysis according to the experimental conditions, and this stimulation was related to the mitochondrial intactness. The ADPase activity in intact mitochondria is 100-fold higher in presence of K +, succinate and 1 mM Mg 2+ while this activity is only increased by two-fold on the SMP when compared to the sample without Mg 2+. It is clearly demonstrated that up-regulation of these enzyme activities occur in intact mitochondria and not on the enzyme itself. The results suggest that the regulation of ATP and ADP hydrolysis is complex, and Mg 2+ plays an important role in the modulation of the extramitochondrial ATPase and ADPase activities in HPM
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