Differential Effects of Early-Life and Postweaning Galacto-oligosaccharide Intervention on Colonic Bacterial Composition and Function in Weaning Piglets.

Abstract

Recently, we proved that the early-life galacto-oligosaccharides (GOS) intervention could improve the colonic function by altering the bacterial composition in suckling piglets. However, whether the early-life GOS (ELG) intervention could have a long influence on the colonic microbiota and whether the combined ELG and postweaning GOS (PWG) intervention would have an interacting effect on maintaining colonic health in weaning piglets remain to be explored. In this study, we illustrated the differential effects of the ELG and PWG interventions on colonic microbiota and colonic function of weaning piglets. Our results showed that the ELG and PWG interventions decreased the frequency of diarrhea in weaning piglets while the PWG intervention increased colonic indexes. After 16S rRNA gene MiSeq sequencing of the gut bacteria belonging to different colonic niches (mucosa and digesta), the increase in the α-diversity of the colonic mucosal bacteria during PWG intervention was revealed. In addition, we found that both the ELG and PWG interventions enriched the relative abundances of short-chain fatty acid (SCFA) producers in different colonic niches and increased the total SCFA concentration in colonic digesta. These changes selectively modulated the mRNA expression levels of pattern recognition receptors and barrier proteins in the colonic mucosa. Of note, the combined effect of ELG and PWG effectively enhanced colonic SCFA producer enrichment and upregulated the butyrate concentration. Meanwhile, the expression levels of MyD88-NF-κB signaling and the proinflammatory cytokines were markedly reduced under the combined effect of ELG and PWG. IMPORTANCE Reducing the disorders of the gut ecosystem is an effective way to relieve weaning stresses of piglets and minimize economic losses in the modern swine industry. To this end, prebiotics have been often added to their diet during the weaning transition. In the present study, we demonstrated that the ELG and PWG interventions showed different effects on the bacterial composition of different colonic niches and on colonic function in the weaning piglets. Especially under the combined effect of ELG and PWG intervention, the expression levels of MyD88-NF-κB and the proinflammatory cytokines decreased with increasing concentrations of butyrate, which is an important microbial metabolite involved in the colon of weaning piglets. These findings further provided new insights into nutritional interventions that alleviate intestinal ecosystem dysbiosis and gut dysfunction in the piglets during the weaning transition.