Differential effects of distal and proximal nerve lesions on carbonic anhydrase activity in rat primary sensory neurons, ventral and dorsal root axons.

Abstract

The effect of proximal and distal peripheral nerve injuries on the histochemistry of carbonic anhydrase (CA) in rat dorsal root ganglion (DRG) neurons, and myelinated (MyF) dorsal and ventral root fibers was studied. Sciatic neurectomy induced no change. Contrariwise, 7 days after lumbar spinal nerve section the numbers of CA-stained ventral root MyF and DRG cells at the L4 and L5 levels decreased to 73.2% and 51.9% of their original values respectively, although the numbers returned to normal by the 90th postoperative day. Dorsal root MyF followed a similar trend, albeit with some delay. Major morphological changes comprised atrophy of dorsal root sensory neurons and axons, particularly in long term experiments, as well as nuclear eccentricity in DRG neurons. These results suggest that, depending on the site of lesion, the rat peripheral nervous system (PNS) either maintains or quickly restores its capacity to synthesize CA. They stand in contrast to the long-lasting metabolic dysfunctions reported to occur when primary neurons are disconnected from the periphery. It is uncertain whether this difference is due to the critical role of CA in neuronal metabolism.