Abstract
Abstract The cis and trans stereoisomers of 3-methyl-dl-proline appeared to inhibit actinomycin synthesis in Streptomyces antibioticus by different mechanisms. Inhibition by trans-3-methyl-dl-proline was reversed by chloramphenicol. The antagonistic effects of cis-3-methyl-dl-proline were prevented or partially reversed by chloramphenicol during the first 2 hours after addition of the inhibitor but became irreversible after this time period. As indicated by experiments in which tritiated imino acid was used, the trans, but not the cis, stereoisomer was incorporated into actinomycins. In both cases the protein fraction was labeled.
Highlights
The antagonistic effects of cis-3-methyl-DL-proline were prevented or partially reversed by chloramphenicol during the first 2 hours after addition of the inhibitor but became irreversible after this time period
Attention was directed toward the possibility that through the use of inhibitors of protein synthesis, which would make available more endogenous amino acid for the synthesis of actinomycin, reversal of 3-Me-proline action might be observed
In the present study the ability of chloramphenicol, as an inhibitor of protein synthesis, to reverse the effects of both cis- and truns-3-Me-proline has been used as a means to determine the nature of the inhibition brought about by either analogue
Summary
LabeledlC-L-prolme (specificactivity, 202mC per mmole) was obtained from New England Nuclear. Streptmnyces antibioticus (ATCC 14888;Rutgers Institute of Microbiology Culture Collection3720)wascultured accordingto Katz, Pienta, and Sivak [1]. All of the experimentsdescribed werecarried out with 100~mcl ulturesof 4% to 72-hour-oldmyCelia. Unlessotherwisespecified,3-Me-proline wasaddedat a concentrationof 1 pg per ml, cti-3-Me-proline at 0.23pg per ml, Efects of S-Methylproline on Actinomycin Biosynthesis Vol 243, No 8 trans-PRO(J- ME)fl. 3-Me-proline (13 mg) and 3H-3-Me-uroline (6.72 X lo cpm,
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