Abstract
The aim of this work was to study whether soluble factors produced in stimulated lymphocyte cultures play any role in the events in the cultures where they have been produced. When the whole culture medium in PPD-stimulated lymphocyte cultures was removed, either daily or on day 3, and replaced by fresh medium with the same antigen concentration, a marked decrease in thymidine uptake resulted. The same operation in PHA-stimulated cultures resulted in an increased thymidine uptake. Suitable controls showed that these effects were not due to artifacts, caused by manipulation of the cultures. If the old medium was replaced by medium from other stimulated lymphocyte cultures, the response was restored to normal levels. Medium from unstimulated cultures had a slight effect, which was considerably increased by addition of PPD. The findings may be related to unspecific conditioning factors, but possibly also to specifically lymphocyte-activating material.
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