Abstract
Background: Avian leukosis virus subgroup J (ALV-J) is an oncogenic virus that causes serious economic losses in the poultry industry; unfortunately, there is no effective vaccine against ALV-J. DNA methylation plays a crucial role in several biological processes, and an increasing number of diseases have been proven to be related to alterations in DNA methylation. In this study, we screened ALV-J-positive and -negative chickens. Subsequently, we generated and provided the genome-wide gene expression and DNA methylation profiles by MeDIP-seq and RNA-seq of ALV-J-positive and -negative chicken samples; 8,304 differentially methylated regions (DMRs) were identified by MeDIP-seq analysis (p ≤ 0.005) and 515 differentially expressed genes were identified by RNA-seq analysis (p ≤ 0.05). As a result of an integration analysis, we screened six candidate genes to identify ALV-J-negative chickens that possessed differential methylation in the promoter region. Furthermore, TGFB2 played an important role in tumorigenesis and cancer progression, which suggested TGFB2 may be an indicator for identifying ALV-J infections.
Highlights
As a member of the Alpharetrovirus genus, avian leukosis virus (ALV) causes different pathotypes of neoplastic diseases in chickens [1, 2]
The results showed that we identified the ALV-Jnegative chickens and Avian leukosis virus subgroup J (ALV-J)-positive chickens
To validate the MeDIP-seq data, we mainly focused on the analysis of differentially methylated regions (DMRs) located in the promoter region, which may be related to gene expression, we detected the methylation level of the transforming growth factor beta 2 (TGFB2) gene
Summary
As a member of the Alpharetrovirus genus, avian leukosis virus (ALV) causes different pathotypes of neoplastic diseases in chickens [1, 2]. The spread of ALVs leads to chicken slow growing, production performance degradation and caused serious losses to the poultry industry [3]. According to their host range and viral envelope protein, ALVs can be classified as ALV-A, -B, -C, -D, -E, -J, and -K subgroups [4, 5]. Avian leukosis virus subgroup J (ALV-J) is an oncogenic virus that causes serious economic losses in the poultry industry; there is no effective vaccine against ALV-J. TGFB2 played an important role in tumorigenesis and cancer progression, which suggested TGFB2 may be an indicator for identifying ALV-J infections
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
