Differential distribution of KCC2 along the axo-somato-dendritic axis of CA1 pyramidal cells

Abstract

Event Abstract Back to Event Differential distribution of KCC2 along the axo-somato-dendritic axis of CA1 pyramidal cells Rita Baldi1*, Csaba Varga1 and Gabor Tamas1 1 Hungarian Academy of Sciences, University of Szeged, Hungary The neuron-specific K-Cl cotransporter (KCC2) plays a crucial role in adjusting intracellular chloride concentrations. Thus, the relative abundance of KCC2 along neuronal plasma membrane compartments can influence the reversal potential of chloride and GABAA receptor mediated synapses as shown for perisomatic and axo-axonic GABAergic synapses. We reported the lack of KCC2 in the axon compared to the soma in the cerebral cortex and extended the quantification of KCC2 to the dendrites of CA1 pyramidal cells with high resolution preembedding immunolocalization. Our results show that KCC2 has an uneven distribution profile along the axo-somato-dendritic axis of CA1 pyramidal neurons. Confirming our results on neocortical pyramidal cells, the axon initial segment contained ~ 5 % of somatic KCC2 cocentrations on CA1 pyramids. Compared to the proximal apical dendrites, the concentration of KCC2 decreased by ~ 37 % in the distal part of stratum radiatum (p<0.0001). Interestingly, the density of gold particles indicating KCC2 concentration was higher in lacunosum moleculare than in the distal part of stratum radiatum, but still ~ 22 % lower than in the proximal dendritic membranes (p<0.03). Basal dendrites showed KCC2 concentrations similar to proximal dendrites of str. radiatum and str. lacunosum moleculare and thus basal dendrites contained more KCC2 than dendrites in the distal str. radiatum. In conclusion, KCC2 is present in the soma as well as in the basal and apical dendritic shafts and spines of CA1 pyramidal cells. Moreover, the density of KCC2 seems to correlate with the relative abundance of GABAergic synapses innervating different segments of the apical dendrites in CA1 pyramidal cells. Conference: 12th Meeting of the Hungarian Neuroscience Society, Budapest, Hungary, 22 Jan - 24 Jan, 2009. Presentation Type: Poster Presentation Topic: Research on the cerebral cortex and related structures Citation: Baldi R, Varga C and Tamas G (2009). Differential distribution of KCC2 along the axo-somato-dendritic axis of CA1 pyramidal cells. Front. Syst. Neurosci. Conference Abstract: 12th Meeting of the Hungarian Neuroscience Society. doi: 10.3389/conf.neuro.01.2009.04.187 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 06 Mar 2009; Published Online: 06 Mar 2009. * Correspondence: Rita Baldi, Hungarian Academy of Sciences, University of Szeged, Szeged, Hungary, baldi.rita@gmail.com Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Rita Baldi Csaba Varga Gabor Tamas Google Rita Baldi Csaba Varga Gabor Tamas Google Scholar Rita Baldi Csaba Varga Gabor Tamas PubMed Rita Baldi Csaba Varga Gabor Tamas Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.