Abstract
Exosome production from cancer-associated fibroblasts seems to be an important driver of tumor progression. We report the first in-depth biotype characterization of ncRNAs, analyzed by Next Generation Sequencing and Bioinformatics, expressed in established primary human normal and cancer-associated fibroblasts (CAFs) from cancer and normal mucosa tissues from 9 colorectal cancer patients, and/or packaged in their derived exosomes. Differential representation and enrichment analyses based on these ncRNAs revealed a significant number of differences between the ncRNA content of exosomes and the expression patterns of the normal and cancer-associated fibroblast cells. ncRNA regulatory elements are specifically packaged in CAF-derived exosomes, supporting a specific cross-talk between CAFs and colon cancer cells and/or other stromal cells, mediated by exosomes. These sncRNAs are potential biomarkers present in cancer-associated fibroblast-derived exosomes, which should thereby contribute to developing new non-invasive diagnostic, prognostic and predictive methods for clinical applications in management of cancer patients.
Highlights
Exosome production from cancer-associated fibroblasts seems to be an important driver of tumor progression
Identification and quantification of Non-Coding RNA (ncRNA) biotypes in cell and exosomal fractions from human primary normal and cancer-associated fibroblasts Primary Normal fibroblast (NF) and Cancer associated fibroblast (CAF) were propagated from 9 colorectal cancer patients (Additional file 1)
RNA samples from CAF and NF derived exosomes were combined to get 3 pools of NF-derived exosomes consisting of 3 samples each (NF-EXO samples) vs 3 pools of CAF-derived exosomes consisting of 3 samples each (CAF-EXO samples)
Summary
Mercedes Herrera1,2, Carlos Llorens3, Marta Rodríguez4,5, Alberto Herrera1, Ricardo Ramos6, Beatriz Gil1,7, Antonio Candia8, María Jesús Larriba9, Pilar Garre10, Julie Earl11, Mercedes Rodríguez-Garrote11, Trinidad Caldés10, Félix Bonilla12, Alfredo Carrato11, Vanesa García-Barberán1,10* and Cristina Peña1,11*
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