DIFFERENTIAL DIAGNOSIS OF ORGANIC AND FUNCTIONAL PATHOLOGY BOWELS ON THE BASIS OF FECAL LACTOFERRIN AND IMMUNOCHEMICAL HEMOCCULT TEST

Abstract

The aim of the study was to assess the possibility of differential diagnosis of organic and functional bowels pathology in the comprehensive comparative analysis of the diagnostic value of clinical signs before the symptoms of anxiety, ESR, and immunochemical tests for fecal occult blood and fecal lactoferrin. 90 patients with organic pathology of intestines (42 patients with active ulcerative colitis and 48 patients with colorectal cancer) and 46 patients with irritable bowel syndrome have been examined. Lactoferrin was detected in stool samples taken from one defecation act, enzyme immunoassay (ELISA) using kits ELISA TEST KIT Hycult Biotechnology (Netherlands). The concentration of the marker at the level of 15,25 mg/g was defined with point of separation. The presence of hemoglobin in the feces was determined using immunochemical test for occult blood in the stool sets firm «Biotech Atlantic, Inc.» (U.S.). Fecal lactoferrin (0,931 ± 0,021) and immunochemical tests for fecal occult blood (0,922 ± 0,0224) showed the highest diagnostic utility in the differential diagnosis of organic and functional pathology of the intestines. They are ahead of the symptoms of anxiety (p = 0,002 and p = 0,005 respectively) and erythrocyte sedimentation rate (p < 0,001 and p = 0,001 respectively). The sensitivity of fecal lactoferrin was 85.56 % (95 % CI: 70,80-87,30), specificity - 84,78 % (95 % CI: 71,10-93,60), immunochemical test for occult blood in feces - 84,44 % (95 % CI: 75,30-91,20) and 100,00 % (95 % CI: 92,20-100,00), respectively. Consequently, fecal lactoferrin and immunochemical hemoccult test were sensitive and specific markers in the differential diagnosis of organic and functional pathology of the intestines.