Differential developmental and proinflammatory responses of zebrafish embryo to repetitive exposure of biodigested polyamide and polystyrene microplastics

Abstract

Microplastics (MPs) have attracted global concern and are at the forefront of current research on environmental pollution, whereas, little is known about the degradation of ingested MPs in the gastrointestinal environment and repetitive exposure-associated risk of egested MPs to organisms. The present study revealed that polyamide (PA) and polystyrene (PS) MPs exhibited remarkably differential biodegradations in the gastric and intestinal fluids of a model fish (Siniperca chuatsi). Significant disintegration of the skeleton structure, size reduction (from 27.62 to 9.17 µm), benzene ring scission, and subsequent biogenic corona coating and surface oxidation occurred during in vitro digestion, thus increasing the hydrophilicity and agglomeration of PS. Conversely, PA MPs exhibited high resistance to enzymolysis with slight surface erosions and protein adsorption. Relative to the pristine form, the bioaccumulation of digested PS elevated and the musculoskeletal deformity and mortality of juvenile zebrafish were obviously enhanced, but these changes were unobservable for PA. Lipopolysaccharide-triggered inflammation and apoptosis via Toll-like receptor signaling pathways and reduction of extracellular matrix secretions driven by oxidative stress contributed to the aggravated inhibitory effects of digested PS on larval development. These findings emphasize the necessity of concerning the biota digestion in MP risk assessments in natural waters.