Abstract
Long-chain bases, found in the oral cavity, have potent antimicrobial activity against oral pathogens. In an article associated with this dataset, Poulson and colleagues determined the cytotoxicities of long-chain bases (sphingosine, dihydrosphingosine, and phytosphingosine) for human oral gingival epithelial (GE) keratinocytes, oral gingival fibroblasts (GF), dendritic cells (DC), and squamous cell carcinoma (SCC) cell lines [1]. Poulson and colleagues found that GE keratinocytes were more resistant to long-chain bases as compared to GF, DC, and SCC cell lines [1]. In this study, we assess the susceptibility of DC to lower concentrations of long chain bases. 0.2–10.0 µM long-chain bases and GML were not cytotoxic to DC; 40.0–80.0 µM long-chain bases, but not GML, were cytotoxic for DC; and 80.0 µM long-chain bases were cytotoxic to DC and induced cellular damage and death in less than 20 mins. Overall, the LD50 of long-chain bases for GE keratinocytes, GF, and DC were considerably higher than their minimal inhibitory concentrations for oral pathogens, a finding important to pursuing their future potential in treating periodontal and oral infections.
Highlights
Differential cytotoxicity of long-chain bases for human oral gingival epithelial keratinocytes, oral fibroblasts, and dendritic cells
In an article associated with this dataset, Poulson and colleagues determined the cytotoxicities of long-chain bases for human oral gingival epithelial (GE) keratinocytes, oral gingival fibroblasts (GF), dendritic cells (DC), and squamous cell carcinoma (SCC) cell lines [1]
Poulson and colleagues found that GE keratinocytes were more resistant to long-chain bases as compared to GF, DC, and SCC cell lines [1]
Summary
Sphingosine, dihydrosphingosine, and phytosphingosine were diluted from 0.2– 80.0 mM for cytotoxicity assays in dendritic cells. There is no data in public repository. Long-chain bases sphingosine and dihydrosphingosine are in saliva and have antimicrobial activity against oral pathogens. We determined the toxicity of sphingosine, dihydrosphingosine, and phytosphingosine for GE keratinocytes, GF, DC, and SCC cells. The LD50 of long-chain bases for GE keratinocytes, GF, SCC cells, and DC were considerably higher than their MIC for oral pathogens. This finding is important in pursuing the future potential of long-chain bases in treating periodontal and oral infections. Sphingosine (D-sphingosine), dihydrosphingosine (D-erythro-dihydrosphingosine), and phytosphingosine were prepared as recently described [1] and diluted in PBS to 640.0 μM stock solutions
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