Differential colonization and functioning of microbial community in response to phosphate levels

Abstract

Microbes play a major role in phosphate cycling and regulate its availability in various environments. The metagenomic study highlights the microbial community divergence and interplay of phosphate metabolism functional genes in response to phosphate rich (100 mgL−1), limiting (25 mgL−1), and stressed (5 mgL−1) conditions at lab-scale bioreactor. Total five core phyla were found responsive toward different phosphate (Pi) levels. However, major variations were observed in Proteobacteria and Actinobacteria with 33–81% and 5–56% relative abundance, respectively. Canonical correspondence analysis reflects the colonization of Sinorhizobium (0.8–4%), Mesorhizobium (1–4%), Rhizobium (0.5–3%) in rich condition whereas, Pseudomonas (1–2%), Rhodococcus (0.2–2%), Flavobacterium (0.2–1%) and Streptomyces (0.3–4%) colonized in limiting and stress condition. The functional profiling demonstrates that Pi limiting and stress condition subjected biomass were characterized by abundant PQQ-Glucose dehydrogenase, alkaline phosphatase, 5′-nucleotidase, and phospholipases C genes. The finding implies that the major abundant genera belonging to phosphate solubilization enriched in limiting/stressed conditions decide the functional turnover by modulating the metabolic flexibility for Pi cycling. The study gives a better insight into intrinsic ecological responsiveness mediated by microbial communities in different Pi conditions that would help to design the microbiome according to the soil phosphate condition. Furthermore, this information assists in sustainably maintaining the ecological balance by omitting excessive chemical fertilizers and eutrophication.