Differential Changes in Expression of Metabolic-related Neuropeptides in the Rat Brain during Morphine Withdrawal-induced Anorexia

Abstract

Event Abstract Back to Event Differential Changes in Expression of Metabolic-related Neuropeptides in the Rat Brain during Morphine Withdrawal-induced Anorexia B. Pintér-Kübler1, S. Ferenczi1, A. Molnár1, C. Núñez2, Z. Szőke1, M. V. Milanés2 and K. J. Kovács1* 1 Institute of Experimental Medicine, Hungary 2 University of Murcia, Spain In this study we followed the effects of chronic morphine exposure and naloxone-precipitated morphine withdrawal on energy balance, metabolic related hormone secretion as well as on the transcription of anorexigenic and orexigenic neuropeptides within the hypothalamus. Animals were implanted with morphine pellets and on day 8, morphine withdrawal was precipitated by an injection of naloxone. Rats were decapitated at various time intervals after injection. Food consumption and the body weight change were recorded daily. Morphine treated animals consumed significantly less food than placebo implanted controls and it was accompanied by a reduction in body weight gain. Morphine withdrawal resulted in elevation of CRH and UCNII expression and a decline in POMC levels. The relative quantity of POMC and CART mRNAs in the hypothalamic samples gradually decrease, while NPY expression is only transiently upregulated. Morphine withdrawal results in serious affective and somatic symptoms including activation of the hypothalamo-pituitary-adrenocortical (HPA) axis. The plasma levels of ACTH were increased and lasted up to 2 hours after administration of naloxone.The present data suggest a possible role of hypothalamic CRH and UCNII,- rather than POMC/CART system in mediation of metabolic changes such as weight loss during naloxone precipitated morphine withdrawal in rats. Keywords: Behaviour and cognition, Neuroscience Conference: 13th Conference of the Hungarian Neuroscience Society (MITT), Budapest, Hungary, 20 Jan - 22 Jan, 2011. Presentation Type: Abstract Topic: Behaviour and cognition Citation: Pintér-Kübler B, Ferenczi S, Molnár A, Núñez C, Szőke Z, Milanés MV and Kovács KJ (2011). Differential Changes in Expression of Metabolic-related Neuropeptides in the Rat Brain during Morphine Withdrawal-induced Anorexia. Front. Neurosci. Conference Abstract: 13th Conference of the Hungarian Neuroscience Society (MITT). doi: 10.3389/conf.fnins.2011.84.00021 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 03 Mar 2011; Published Online: 23 Mar 2011. * Correspondence: Dr. K. J Kovács, Institute of Experimental Medicine, Budapest, Hungary, kovacs@koki.hu Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers B. Pintér-Kübler S. Ferenczi A. Molnár C. Núñez Z. Szőke M. V Milanés K. J Kovács Google B. Pintér-Kübler S. Ferenczi A. Molnár C. Núñez Z. Szőke M. V Milanés K. J Kovács Google Scholar B. Pintér-Kübler S. Ferenczi A. Molnár C. Núñez Z. Szőke M. V Milanés K. J Kovács PubMed B. Pintér-Kübler S. Ferenczi A. Molnár C. Núñez Z. Szőke M. V Milanés K. J Kovács Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.