Abstract
Rat gastric mucosal cells were separated according to size by rate-zonal sedimentation through a medium containing a gradient of bovine albumin. Numbers and mean sizes of cells indicated two main populations, 430-1000 microns 3 and 1500-2500 microns 3, with a discontinuity between 1000 and 1500 microns 3. Gradient fractions, combined into pools of increasing cell size, synthesized radiolabelled proteins which were resolved by electrophoresis, densitometry and computation. The smallest cells (430-800 microns 3) tended to synthesize radiolabelled proteins with the highest relative specific radioactivities. The decrease in relative specific radioactivity with increase in cell size was compatible with a progression from precursor cells towards the more differentiated state.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
More From: Comparative biochemistry and physiology. B, Comparative biochemistry
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.