Abstract
Ex situ bioremediation of crude oil in bioreactors by oleophilic bacteria permits to regulate temperature, nutrients and oxygen and the results in petroleum removal are much more effective than in situ bioremediation. This article quantify the biodegradation of alkanes which conform crude oil in bioreactors by three oleophilic strains. Samples were collected from the bioreactors and extracted in dichloromethane (10:1 aqueous/organic v/v) for amplification in GC-FID analysis of individual alkanes. 94% of crude oil (initial concentration 8.6 g l−1) is removed in 24 days by Bacillus licheniformis and in 38 days by Pseudomonas putida and Paenibacillus glucanolyticus. Higher biodegradation rates (9.0–26.5 mg l−1d−1) are reached for the lightest fraction of crude oil by Bacillus licheniformis (C10–C16), and much lower for C22–C25 alkanes (4.4–8.9 mg l−1d−1). Pseudomonas putida and Paenibacillus glucanolyticus degrade a wide range of alkanes at 1.6–9.6 mg l−1d−1, with preference over C8–C16 hydrocarbons. Heptane and octane (alkanes cited to be toxic) are difficult for biodegradation in two strains (B. licheniformis and P. putida) and a recalcitrant “window” C18–C21 is observed for the three oleophilic strains in the alkane range studied.
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