Differential Assessments of Blood Lipids, Lipid Peroxidation, Antioxidant Status, and Fatty Acids in Women with Uterine Tumors during Menopausal Period

Abstract

ObjectiveTo study alterations in lipid spectrum, lipid peroxidation intensity, and fatty acid (FA) spectrum in blood of women with benign & malignant uterine tumors during menopausal period.MethodsBlood samples from benign (myoma, n=15) and malignant (n=15) cases were collected, along with healthy menopausal (control, n=15). Spectrophotometric methods assessed lipid spectrum composition, antioxidant enzymes activities & lipid peroxidation intensity. Quantitative analyses of FA were performed by gas‐liquid chromatography.ResultsSaturated FA in malignant cases: decreased myristic acid (3‐fold), palmitic acid (~1.3 fold) compared to control, but stearic acid increased (1.13‐fold). The decreased ratio between palmitic/stearic acids (C16:0/C18:0) by ~1.5‐times was noted. Unsaturated FA in malignant cases showed a percent decrease in oleic (2.9‐fold), linolenic (1.5‐fold), eicosatrienoic (3‐fold), arachidonic (1.3‐fold) acid compared to control. The increased ratio between stearic/oleic acids (C18:0/C18:1) by 3.3‐times was noted in malignant cases. Increase in lipid peroxidation intensification was associated with lipid spectrum composition (hyperlipidemia, disruption in the exchange rate of phospholipids). The negative correlation between lipid peroxidation intensity & phospholipids was r=−0.96±0.04. Total cholesterol was higher in malignant cases. Total amount & activities of superoxide dismutase (SOD), catalase (CAT) decreased in malignant cases. The negative correlation between total cholesterol & SOD, CAT activities was r=−0.99±0.014. Amine‐containing phospholipids decreased but choline‐containing phospholipids increased with the severity of the disease.ConclusionsIntensification of lipid peroxidation & alterations in lipid spectrum composition revealed hyperlipidemia, disruption of phospholipids' exchange rate and hypercholesterolemia. Thus, specific changes in these biochemical parameters can be useful in the differentiation of benign and malignant uterine tumors for menopausal women.This abstract is from the Experimental Biology 2019 Meeting. There is no full text article associated with this abstract published in The FASEB Journal.