Abstract
We report a novel method for preparing electrode surfaces that present thiol-containing or thiol-reactive groups using a single organic silane linkage terminated with 1,3-dithiane via differential anodic oxidation of 1,3-dithiane. We found that the surface groups resulting from the anodic oxidation reaction differed according to the concentration of H2O in the electrolyte solution during the oxidation reaction. The differential anodic oxidation was used successfully for the orthogonal immobilization of two different proteins. We demonstrated also the preparation of an immunoassay platform via orthogonal immobilization, which was then used to detect multiple target antigens based on a sandwich immunoassay.
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