Abstract
The adsorption of wild cucumber mosaic virus by magnesium bentonite showed a marked dependence on pH and magnesium ion concentration. At a concentration of Mg ++ of 5 × 10 −4 M, the adsorption of each component of the virus (0.5 mg/ml) by bentonite (5 mg/ml) changed from almost zero at pH 7.55 to 100% at pH 6.86. Under certain conditions of pH and Mg ++ concentration a differential existed in the adsorption of the two components of the virus. In our tests this differential was maximum at pH 7.15 and a Mg ++ concentration of 5 × 10 −4 M when 100% of the top component and only 15% of the bottom component was adsorbed. Under salt conditions which led to differential adsorption of the components, their electrophoretic mobilities were different. As the particles adsorbed onto bentonite could be approximately quantitatively eluted from the bentonite by means of Na 3EDTA, the differential adsorption suggests a convenient method of separating the two components. Tests carried out on turnip yellow mosaic virus gave no indication of a differential in adsorption between the two components of this virus.
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