Differential Action of trans-10, cis-12 Conjugated Linoleic Acid on Adipocyte Differentiation of Ovine and 3T3-L1 Preadipocytes

T Iga,S H Song,K Fukui,S Sasaki,K C Choi,S G Roh,T Satoh,S Yamamoto

doi:10.5713/ajas.2009.80475

Abstract

Trans-10, cis-12 conjugated linoleic acid (CLA) has been reported to inhibit the adipocyte differentiation of preadipocytes in non-ruminant animals (mice, rat, and human). However, the effects of trans-10, cis-12 CLA have not been clear in ruminants. The objective of this study was to investigate the effects of trans-10, cis-12 CLA on adipocyte differentiation of ovine preadipocytes. Differentiation of these preadipocytes was facilitated by treatment with trans-10, cis-12 CLA. Trans-10, cis-12 CLA increased the number and size of oil red O-stainable lipid drops as well as the levels of GPDH activity. PPAR-γ2 and adipophilin mRNA, adipogenic marker genes, were increased by treatment with trans-10, cis-12 CLA. This result was different from that observed with 3T3-L1 preadipocytes, a clonal cell line derived from rodents. Furthermore, trans-10, cis-12 CLA alone induced the adipocyte differentiation of ovine preadipocytes in differentiation-induction medium without troglitazone. These results suggest that CLA is an inducer and regulator in adipocyte differentiation of ovine preadipocytes, with species differences between ovine and rodent preadipocytes.

Highlights

Dietary products and meat from ruminant animals are important sources of energy, nutrients, and a variety of vitamins
Sci. 22(11):1566-1573 expression of PPAR-γ2 mRNA and the several mRNA levels that are involved in adipocyte differentiation, including CCAAT/enhancer-binding protein-α (C/EBP-α), sterol regulated element binding protein 1-c (SREBP-1c), and PPAR-γ2 target genes such as fatty acid binding protein, perilipin-A (Chung et al, 2005b), lipoprotein lipase (LPL), and adiponectin (Brown and McIntosh, 2003; House et al, 2005)
Ovine preadipocytes treated with trans10, cis-12 conjugated linoleic acid (CLA) had more differentiated adipocytes with large droplets compared with the control (Figure 1a)

Summary

Introduction

Dietary products and meat from ruminant animals are important sources of energy, nutrients, and a variety of vitamins. Ruminant products such as milk, cheese, and beef contain some fat, with these fats being composed of many different types of fat and fatty acids. One of these fatty acids is conjugated linoleic acid (CLA) (Griinari et al, 2000; Daniel et al, 2004). CLA is produced as a result of bacterial biohydrogenation of lipids in the ruminant gut (Mir et al, 2004). CLAs are a group of polyunsaturated fatty acids. Sci. 22(11):1566-1573 expression of PPAR-γ2 mRNA and the several mRNA levels that are involved in adipocyte differentiation, including CCAAT/enhancer-binding protein-α (C/EBP-α), sterol regulated element binding protein 1-c (SREBP-1c), and PPAR-γ2 target genes such as fatty acid binding protein (aP2), perilipin-A (Chung et al, 2005b), lipoprotein lipase (LPL), and adiponectin (Brown and McIntosh, 2003; House et al, 2005)

Objectives

Methods

Results

Conclusion