Abstract
We have isolated two closely related genes, Sbe2.1 and Sbe2.2, encoding isoform II of starch branching enzyme (SBE) from an Arabidopsis thaliana genomic library. Although a partial cDNA clone encoding the isoform I of the potato SBE was used as probe, no clones corresponding to this isoform were found in the Arabidopsis library. Sbe2.1 was completely and Sbe2.2 partially sequenced. PCR-screening of a large number of individual plants using gene specific primers, revealed that both genes were independently present in the genome of A. thaliana. The Sbe2.1 gene consisted of 18 exons interrupted by 17 introns and the open reading frame comprised 2574 bp encoding a protein with a calculated molecular mass of 97 660 Da. The promoter region of both Sbe2 genes contained potential sucrose responsive elements (SURE1 and SURE2). In addition, a potential light responsive element (G-box) was present in the Sbe2.1 promoter. Northern blot analysis using gene specific probes, showed a differential accumulation of transcripts from the Sbe2 genes as a response to light. While the level of Sbe2.1 transcripts increased and decreased during light and dark, respectively, those transcribed from the Sbe2.2 gene did not change significantly. However, transcripts from both Sbe2 genes accumulated strongly when plants were incubated in light and with exogenous supply of either glucose, fructose or sucrose. No significant changes in Sbe2 transcript levels were detected in plants incubated with sorbitol under conditions of dark or light, indicating that the expression of Sbe2 genes is stimulated by specific carbohydrate signals.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.