Different Th1 and Th2 citokine profile in serum and tumor microciculation of SCLC and NSCLC patients

Abstract

Th1 type is impotrant in anti tumor response, while Th2 negatively affects protective response. Objective was to investigate key Th1 and Th2 cytokines both in tumor microenvironment and serum samples, and compare cytokine profiles between patients with lung tumors of different histological characteristics. The study included 41 lung tumor patients in III and IV clinical stage. IL-2, IL-12, IFN-gama, IL-4, IL-5, IL-6, IL-10, and IL-13. Comparative analysis of serum cytocines in lung cancer patients demonstrated that group with SCLC tumors had significant IL-6 and IL-13 concentration. NSCLC group of patients showed different dominant cytokine profiles. Samples of patient with NSCLC Ad contained high levels of IL-2, IFN-gama, IL-4, IL-10, and IL-10, patients with NSCLCSq had high IL-6 and IL-13, while patients with NSCLC LC had high IL-2 and IFN-gama. Citokine profile in direct tumor microenvironment, tumor microcirculation, was completely different from profile estimated in sera samples. Tumor samples from SCLC patients were characterized with high IL-12, IFN-gama, IL-6 and IL-13. NSCLC Ad patients had high IFN-gama, IL-6 and IL-13, NSCLC SQ patients had high IL-6 and IL-13, while NSCLC LC had high IL-5 and IL-13. Analysis of investigated cytokines in both compartmens of same patient revealed distinct with SCLC tumors had significatly increased IL-2, IL-12 and IL-6, NSCLC Sq had increased IL-12 and IL-5, while NSCLC LC had significantly increased IL-5 and IL-13 tumor/serum ratio. Lung tumors with different histological characteristics differ signficantly among themselvels in the aspect of detected Th1 and Th2 cytokine profile.