Abstract
Binding of the glucocorticoid dexamethasone was studied in intact cells of the mouse lymphoma lines S49. 1, WEHI-7, WEHI-22, and WEHI-112. The number of binding sites per cell varied from 13 000 to 130 000 depending on the cell line. The equilibrium dissociation constant at 37° was in the range of 10 nM. When dexamethasone binding was investigated at 0° in cytosol preparations of the same cell lines significantly lower receptor levels were found and the dissociation constants were about one order of magnitude lower than those determined in whole cells. The data suggest that glucocorticoid receptors exist in different states in intact cells and cell extracts.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callMore From: Biochemical and Biophysical Research Communications
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
