Different Responses of Photosynthesis to Nitrogen Starvation Between Highly Oil-Accumulative Diatoms, Fistulifera solaris and Mayamaea sp. JPCC CTDA0820.

Abstract

Highly oil-accumulative diatoms are expected to be a promising biomass for the production of biofuel. To harvest the diatom oils at high yields, it is critical to elucidate the relationship of oil accumulation with photosynthesis under fluctuating environmental conditions. Here, we characterized the physiological responses of the growth and photosynthesis in the mesophilic diatom Fistulifera solaris and the cold-tolerant one Mayamaea sp. JPCC CTDA0820 to nitrogen starvation, one of the most notable abiotic stresses, where most eukaryotic algae decrease their photosynthetic activity and accumulate oil in the cells. While F. solaris started showing growth retardation at NaNO3 levels less than 50% of a normal F/2 artificial seawater (ASW) medium, Mayamaea sp. sustained normal growth even at a NaNO3 level 10% of normal F/2ASW, indicating the sharp contrast of nitrogen requirement between these two diatom species. In the transition from 100 to 0% nitrogen conditions in the modified F/2ASW, F. solaris showed a clear suppression of chlorophyll (Chl)-based photosynthetic O2 evolution rate and relative electron transport rate at photosystem II, which were negatively correlated to the capacity of non-photochemical quenching. Meanwhile, there was no change in these Chl-based parameters observed in nitrogen-starved Mayamaea sp. Instead, Mayamaea sp. showed a significant decrease in the Chl a amount per cells. These data suggested the occurrence of two types of photosynthetic responses to nitrogen starvation in oleaginous diatoms; that is, (1) suppression of photosynthetic activity per Chl with enhancing heat dissipation of excess light energy and (2) synchronous suppression of cellular photosynthetic activity with Chl amounts.