Different Preclimacteric Events in Apple Cultivars with Modified Ripening Physiology

Vikram Singh,Asya Weksler,Haya Friedman

doi:10.3389/fpls.2017.01502

Vikram Singh, Asya Weksler + Show 1 more

Open Access

https://doi.org/10.3389/fpls.2017.01502

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Abstract

“Anna” is an early season apple cultivar exhibiting a fast softening and juiciness loss during storage, in comparison to two mid-late season cultivars “Galaxy” and “GD.” The poor storage capacity of “Anna” was correlated with high lipid oxidation-related autoluminescence, high respiration and ethylene production rates, associated with high expression of MdACO1, 2, 4, 7, and MdACS1. All cultivars at harvest responded to exogenous ethylene by enhancing ethylene production, typical of system-II. The contribution of pre-climacteric events to the poor storage capacity of “Anna” was examined by comparing respiration and ethylene production rates, response to exogenous ethylene, expression of genes responsible for ethylene biosynthesis and response, and developmental regulators in the three cultivars throughout fruit development. In contrast to the “Galaxy” and “GD,” “Anna” showed higher ethylene production and respiration rates during fruit development, and exhibited auto-stimulatory (system II-like) effect in response to exogenous ethylene. The higher ethylene production rate in “Anna” was correlated with higher expression of ethylene biosynthesis genes, MdACS3a MdACO2, 4, and 7 during early fruit development. The expression of negative regulators of ripening (AP2/ERF) and ethylene response pathway, (MdETR1,2 and MdCTR1) was lower in “Anna” in comparison to the other two cultivars throughout development and ripening. Similar pattern of gene expression was found for SQUAMOSA promoter binding protein (SBP)-box genes, including MdCNR and for MdFUL. Taken together, this study provides new understanding on pre-climacteric events in “Anna” that might affect its ripening behavior and physiology following storage.

Highlights

Apple (Malus × domestica, subfamily; Maloideae, family; Rosaceae) is a classical climacteric fruit, generating an ethylene burst at the onset of ripening concomitantly with an increase in respiration (Alexander and Grierson, 2002; Giovannoni, 2004)
This study was aimed to understand the ripening behavior of “Anna,” by comparing events at pre-climacteric stage to those in “Galaxy” and “Golden Delicious (GD).” Respiration, ethylene production, response to exogenous ethylene, and expression of genes involved in ethylene biosynthesis and response, and developmental regulators were compared between the cultivars at maturity, as well as, during fruit development
Fruit were sliced as a wedge from two opposite sides, peel and core were removed, tissue was frozen in liquid nitrogen and stored at −80◦C, RNA was extracted from three biological replicated, each containing five fruit

Summary

Introduction

Apple (Malus × domestica, subfamily; Maloideae, family; Rosaceae) is a classical climacteric fruit, generating an ethylene burst at the onset of ripening concomitantly with an increase in respiration (Alexander and Grierson, 2002; Giovannoni, 2004). “Anna” exhibits fast ripening, inability to maintain the crisp texture and becomes mealy, and has a poor storage capacity (even at 0◦C) (Klein and Lurie, 1990). The reasons for this peculiar attributes of “Anna” are still not understood and the mechanism of ethylene biosynthesis and response is yet to be identified

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