Different oviposition behaviour in Chrysomelid beetles: Characterisation of the interface between oviposition secretion and the plant surface

Abstract

The behavioural sequence of the oviposition process can be quite distinct in herbivorous leaf beetles: eggs are either connected to the plant surface or laid into mesophyll tissue. Females of two beetle species were recorded with a digital camera in order to analyse their oviposition behaviour. The morphology of the plant-egg-interface was investigated in three species by histological studies and scanning electron microscopy. The lily leaf beetle, Lilioceris lilii, oviposits directly on the plant cuticle without damaging the plant tissue. Several eggs are released together with secretion and are laid in a row. The secretion sorbes in the plant cuticle. In the close relative, Lilioceris merdigera, the secretion can enter the leaf tissue via the stomata. The mustard leaf beetle, Phaedon cochleariae, gnaws a characteristic hole into the epidermis and part of the mesophyll. Into this cavity, it releases in an alternating sequence secretion, a single egg and again secretion. The egg, which is usually laid on plants growing in wetlands, is surrounded by an extrachorion that might possess a respiratory function. The potential eco-physiological relevance of the specific nature of the placement of eggs and secretion on or in the plant tissue is discussed.