Abstract
A common feature of stress signalling pathways are alterations in the concentration of cytosolic free calcium ([Ca2+]cyt), which allow the specific and rapid transmission of stress signals through a plant after exposure to a stress, such as salinity. Here, we used an aequorin based bioluminescence assay to compare the NaCl-induced changes in [Ca2+]cyt of the Arabidopsis ecotypes Col-0 and C24. We show that C24 lacks the NaCl specific component of the [Ca2+]cyt signature compared to Col-0. This phenotypic variation could be exploited as a screening methodology for the identification of yet unknown components in the early stages of the salt signalling pathway.
Highlights
Worldwide, approximately 20% of the irrigated agricultural land is affected by soil salinity [1]
In many crop species salinity stress is predominantly due to high levels of sodium chloride (NaCl) in the soil
Plant salinity stress can be categorised into two phases, the initial shoot ion-accumulation independent stress and the later shoot ion-dependent stress [2,3]
Summary
Approximately 20% of the irrigated agricultural land is affected by soil salinity [1]. To investigate the early stages of the salt signalling pathway, we compared the calcium signatures of Col-0 and C24 using the aequorin bioluminescence reporter system.
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