Different mobile counter ion types of magnetic ion-exchange resin coupling with ozonation treatment of the organic matter, antibiotic resistance genes, and pathogens in secondary effluent

Abstract

Advanced treatment and reuse of secondary effluents (SE) from wastewater treatment plants is considered an effective method of alleviating the conflict between water supply and demand. However, the effective removal of organic matter, antibiotic resistance genes (ARGs), and pathogens in SE is key to ensure the safety of reclaimed water. In this study, the effectiveness of three types of magnetic ion-exchange resins (R-X, where X refers to –OH, –HCO3, and -Cl) coupled with ozonation (R + O) in reducing different types of carbon, pathogens, intracellular and extracellular antibiotic resistance genes (iARGs and eARGs), and microbial communities in SE was investigated. The results indicated that R-Cl was the best pre-treatment resin for ozonation. R-Cl + O (8 mL/L R-Cl adsorption for 30 min, followed by 15 min of 8 mg/L ozonation) achieved removal efficiencies of 49.7 %, 86.6 %, 99.9 %, 87.1 %, and 99.9 % for dissolved organic carbon, UV254, ΣiARGs, ΣeARGs, and Σpathogens, respectively. Ozonation was more effective than resin adsorption in the removal of ARGs, and R-Cl + O had the lowest rebounding potential for iARGs and pathogens. In addition, R-OH had the lowest removal efficiency of organic matter, iARGs, and pathogens compared to other types of resins owing to the electrostatic repulsion mechanism. Molecular dynamics simulations and average reduced density gradient analysis revealed that the quaternary amine sites of the resin had a positive surface electrostatic potential, which could firmly adsorb double-stranded deoxyribonucleic acid by electrostatic action. Ozonation effectively reduced the risk of ARGs and pathogens in the effluent of resin. The R + O treatment reduced organic matter, ARGs, and pathogens more effectively than single treatment, despite the different anionic groups of the resin.