Different Methods for Spectrophotometric Estimation and Validation of Paracetamol and Domperidone in Pure and Tablet Dosage Form

Abstract

Two simple, accurate and precise spectrophotometric methods were developed for simultaneous determination of Paracetamol and Domperidone in combined pharmaceutical dosage form. The first method is Dual wavelength method principle for which is “the absorbance difference between two points on the mixture spectra is directly proportional to the concentration of the component of interest” and second method is Area under curve. In first method wavelengths selected for determination of paracetamol were 278.0 nm and 287.0 nm, whereas, the wavelengths selected for determination of Domperidone were 236.0 nm and 252.0 nm. In second method wavelength ranges of 237.0-251.0 nm and 275.0-289.0 nm were selected to determine PARA and DOM respectively by AUC. 0.5 M Methanolic HCl was taken as a solvent. Regression analysis of Beer’s plots showed good correlation in concentration range of 5-25 μg/ml for PARA and 5-25 μg/ml for DOM. The precision by repeatability of method was found within limits. The proposed method was successfully applied to determination of these drugs in commercial tablets.