Different in cytoskeleton reorganization in tobacco root cells in the original samsun variety and a transgenic line with FeSOD1 overexpression under salinity

Abstract

The aim of the study was to study the state and reaction of cytoskeletal elements - microtubules and actin laments, in root cells of Samsun tobacco plants and its transgenic line expressing the FeSOD1 gene from Arabidopsis thaliana with the pea rbcS leader sequence for the localization of the gene product in chloroplasts encoding Fe-dependent superoxide dismutase, constitutively inducing intracellular oxidative stress, by increasing the H2O2 pool for a long-term e ect of moderate concentrations of NaCl and Na2SO4. The main hypothesis was to identify the positive protective e ect of controlled constant oxidative stress on the stability of the most sensitive system that provides growth by division and growth by extension - the tubulin cytoskeleton and e ective intracellular transport and structural stability - the actin lament system. Localization of the microtubule cytoskeleton and actin laments using antibodies to tubulin clone DM1α and actin clone 10-B3 by transmission electron microscopy and immunocytologically, detected by treatment with the second antibodies conjugated with Alexa-488, made it possible to establish signs of reorganization and disassembly of the actin lament network under the action of NaCl and Na2SO4 as in control and in transgenic plants. At the same time, in transgenic plants, di erences can be noted even without exposure, which indicates the e ectiveness of this method for stimulating a protective response. These data suggest that the state of the system of the tubulin cytoskeleton and actin laments may be an indicator of the resistance of FeSOD1 transgenic plants to salinity. A relationship has also been established between the reorganization of the cytoskeleton and vacuolization, especially with Na2SO4.