Abstract
This boom in research has led its aquaculture to grow exponentially worldwide without sufficient accompanying studies on novel methods for husbandry and larval rearing which would optimize an intensive production of fish for research with adequate standardization and fish welfare. With new transgenic tools being rapidly developed, the zebrafish mutation project as well as its current role as a powerful tool in drug screens, it became essential to find better protocols and methods to breed and raise zebrafish faster, more reliably and with better welfare. However, little is known on the nutritional requirements of zebrafish [29, 30], being mostly reared with information available for Cyprinaformes [30, 31]. This is becoming a major concern within the research community as it imposes a difficulty to standardize a husbandry protocol in different facilities [32, 33]. Proper nutrition is not only important for individual growth and survival [29] but also to the reproductive success [34] which directly affects offspring fitness [35].
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